Nature Methods
Quantifying domain-ligand affinities and specificities by high-throughput holdup assay
Renaud Vincentelli, Katja Luck, Juline Poirson, Jolanta Polanowska, Julie Abdat, Marilyne Blémont, Jeremy Turchetto, Fran?ois Iv, Kevin Ricquier, Marie-Laure Straub, Anne Forster, Patricia Cassonnet, Jean-Paul Borg, Yves Jacob, Murielle Masson, Yves Nominé, Jér?me Reboul, Nicolas Wolff, Sebastian Charbonnier, Gilles Travé.
This paper reports an approach to measure equilibrium binding affinities for interacting proteins in high throughput, allowing the rapid and quantitative profiling of the specificity of interaction motifs.