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Nature Methods

Structural analysis of multicellular organisms with cryo-electron tomography

Jan Harapin, Mandy B?rmel, K Tanuj Sapra, Damian Brunner, Andres Kaech, Ohad Medalia.
Tissues from multicellular organisms can be imaged by cryo-electron tomography using an optimized combination of vitrification, milling and application of fiducial markers.