Nature Methods
In vivo single-cell labeling by confined primed conversion
William P Dempsey, Lada Georgieva, Patrick M Helbling, Ali Y Sonay, Thai V Truong, Michel Haffner, Periklis Pantazis.
Primed conversion of proteins such as Dendra2 confines photoconversion to a limited 3D volume through a sequential absorption process relying on illumination with two separate low-intensity lasers and enables precise targeting of single cells.