Nature Methods
Application of BRET to monitor ligand binding to GPCRs
Leigh A Stoddart, Elizabeth K M Johnstone, Amanda J Wheal, Jo?lle Goulding, Matthew B Robers, Thomas Machleidt, Keith V Wood, Stephen J Hill, Kevin D G Pfleger.
Bioluminescence resonance energy transfer, from the substrate of a luciferase that is fused with a G protein–coupled receptor to a fluorescent dye covalently linked to a receptor ligand, allows the profiling of ligand affinity and binding kinetics.